Projects
Development of Synthetic Methodology and Approaches to Modified Nucleobases, Nucleosides and Nucleotides
Although our group is primarily not aiming at synthetic methodology development, we need to develop new methodologies and approaches for the synthesis of modified nucleobases, nucleosides and nucleotides for the applications in medicinal and bioorganic chemistry projects...
Medicinal chemistry of analogues of nucleobases and nucleosides
In collaboration with the group of Prof. M. Hajduch at the Palacky University Olomouc and with Gilead Sciences, Inc. (Foster City, CA, U.S.A.), we design and synthesize novel modified nucleosides as potential antivirals or cytostatic agent...
Synthesis of modified nucleoside triphosphates and polymerase construction of functionalized nucleic acids
Novel efficient and expeditious two-step approach for the construction of functionalized nucleic acids was developed in our group. It is based on direct modification of nucleoside triphosphates followed by enzymatic incorporation of the modified dNTPs by DNA polymerase...
Fluorescent or redox labelling of DNA for bioanalytical applications
One of the attractive areas of applications of modified nucleic acids is bioanalysis and diagnostics. We focus mainly on fluorescent and redox labelling...
Chemical biology of base-modified nucleic acids
The second area of applications of base-modified nucleic acids is chemical biology. In particular, we are interested in applications of modified DNA in studying or modulation of protein-DNA interactions...